宁夏地区奶牛乳房炎大肠杆菌毒力基因检测和耐药性分析

doi:10.11843/j.issn.0366-6964.2016.01.024

Abstract

Abstract:

The objectives of this study were to investigate resistance，virulence genes and resistance genes of Escherichia coli isolated from dairy cow mastitis in Ningxia.The antimicrobial resistance of 197 Escherichia coli from dairy cow mastitis cases to 16 drugs was determined by the methods of K-B and the virulence genes and resistance genes were detected by PCR.The results showed that the resistance rate of ampicillin was the highest and it achieved 54.69%.Furthermore，the resistance rates of doxycycline，tetracycline and streptomycin were about 39%.It was more sensitive to cephazolin，cefotaxime，polymyxin，florfenicol，quinolones，and the sensitive rates were above 60%.The results of genes detection showed that the detection rates of virulence genes astA，escV，eaeA，sepA，hlyA were 20.30%，14.72%，8.12%，2.03% and 1.02% respectively.The virulence genes sta and stx2e were not detected.Tetracycline resistant genes were detected and the detection rate of tetC was 24.87%.The detection rate of fluoroquinolone resistance genes GyrA，GyrB and ParC were 97.46%，98.98% and 98.48%，respectively.Our study demonstrates Escherichia coli isolates from dairy cow mastitis in Ningxia carrying virulence genes astA，eaeA，sepA，escV，hlyA and the genes mediate resistance to tetracyclines，fluoroquinolones，and those isolates have varying degrees of resistance 16 kinds of antibacterial drugs，and some isolates are multi-resistant strains.

CLC Number:

S852.612

ZHANG Jin-bao，MA Chun-fang，YU Ting，LI Xiao-na，WEI Xiao-ping，WANG Gui-qin. Detection of Virulence Genes and Antimicrobial Resistance Analysis of Escherichia coli Isolated from Dairy Cow Mastitis in Ningxia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 177-182.

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References

［1］杜冬冬.一株猪源大肠杆菌的分离鉴定及耐药性分析［D］.泰安：山东农业大学，2012.
DU D D.Isolation and identification of a porcine Escherichia coli and analysis of drug resistance［D］.Tai’an：Shandong Agricultural University，2012.(in Chinese)
［2］安微，张秀英，李蕊，等.致病性大肠杆菌毒力因子和耐药性研究进展［J］.畜牧与兽医，2013，45(8)：106-110.
AN W，ZHANG X Y，LI R，et al.Research progress on virulence factors and antimicrobial resistance of pathogenic Escherichia coli［J］.Animal Husbandry & Veterinary Medicine，2013，45(8)：106-110.(in Chinese)
［3］张金宝，李晓娜，王桂琴.大肠埃希菌毒力基因的研究进展［J］.动物医学进展，2014，35(8)：70-74.
ZHANG J B，LI X N，WANG G Q.Advance in virulence genes of Escherichia coli［J］.Progress in Veterinary Medicine，2014，35(8)：70-74.(in Chinese)
［4］BUGAREL M，MARTIN A，FACH P，et al.Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains：a basis for molecular risk assessment of typical and atypical EPEC strains［J］.BMC Microbiol，2011，11：142．
［5］杨滴，王耀兵，李冬梅，等.粪便中大肠埃希菌的分离鉴定［J］.微生物学杂志，2007，27(3)：1-5.
YANG D，WANG Y B，LI D M，et al.Isolation and identification of E.coli from feces［J］. Journal of Microbiology，2007，27(3)：1-5.(in Chinese)
［6］Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptbility testing.Twentieth informaitional supplement［S］.CLSI documents M100-S22.CLSI，2012.
［7］ASLANI M M，ALIKHANI M Y，ZAVARI A，et al.Characterization of enteroaggregative Escherichia coli (EAEC) clinical isolates and their antibiotic resistance pattern［J］.Int J Infect Dis，2011，15(2)：136-139.
［8］王宏.牛源大肠杆菌耐药性及耐药机制研究［D］.哈尔滨：东北农业大学，2011.
WANG H.Study on antibiotic resistance and resistant mechanism of bovine E.coli［D］.Harbin：Northeast Agricultural University，2011.(in Chinese)
［9］梁宏儒.黑龙江部分奶牛大肠杆菌耐药性的耐药基因检测分析［D］.大庆：黑龙江八一农垦大学，2013.
LIANG H R.Detection and analysis of resistance and resistance gene of E.coli isolated from part of dairy farms in Heilongjiang procince［D］：Daqing：Heilongjiang Bayi Agricultural University，2013.(in Chinese)
［10］PIDDOCK L J.Mechanism of quinolone uptake into bacterial cells［J］.J Antimicrob Chemother，1991，27(4)：399-403.
［11］BEARDEN D T，DANZIGER L H.Mechanism of action of and resistance to quinolones［J］.Pharmacotherapy，2001，21(10)：224-232.
［12］杨汉春，陈声，将鸿蒙，等.鸡源大肠杆菌对氟喹诺酮类药物的多重耐药性［J］.畜牧兽医学报，2003，34(4)：398-404.
YANG H C，CHEN S，JIANG H M，et al.Multiple fluoroquinolone resistance in clinical isolates of Escherichia coli from chickens［J］.Acta Veterinaria et Zootechnica Sinica，2003，34(4)：398-404.(in Chinese)
［13］HANSEN H，HEISIG P.Topoisomerase IV mutations in quinolone-resistant salmonellae selected in vitro［J］.Microb Drug Resist，2003，9(1)：25-32.
［14］尹召华.奶牛乳腺炎的病原菌分离鉴定、毒力基因检测与基因治疗研究［D］.扬州：扬州大学，2009.
YIN S H.Identification，virulent gene detection and gene therapy of mastitis-causing bacteria in dairy cows［D］.Yangzhou：Yangzhou University，2009.(in Chinese)
［15］杨少华，柴同杰.断奶幼兔腹泻肠致病性大肠杆菌LEE毒力岛的分子检测［J］.中国兽医杂志，2004，40(10)：6-8.
YANG S H，CHAI T J.Molecular epidemiological investigation of LEE pathogenic island of E.coli strains isolated from diarrheic weaned rabbits［J］.Chinese Journal of Veterinary Medicine，2004，40(10)：6-8.(in Chinese)
［16］黄名钱.猪源大肠杆菌四环素耐药基因检测及大肠杆菌抑制剂筛选［D］.南昌：江西农业大学，2013.
HUANG M Q.Detection on tetracycline resistance gene and suppressor screening of Escherichia coli in swine［D］.Nanchang：Jiangxi Agricultural University，2013.(in Chinese)
［17］NEELA F A，NONAKA L，RAHMAN M H，et al.Transfer of the chromosomally encoded tetracycline resistance gene tet(M) from marine bacteria to Escherichia coli and Enterococcus faecalis［J］.World J Microb Biot，2009，25(6)：1095-1101.
［18］刘维红.猪沙门氏菌耐药基因检测方法的建立及其应用［D］.武汉：华中农业大学，2006.
LIU W H.Establishment of detection methods of resistant genes for swine Salmonella and the application［D］.Wuhan：Huazhong Agricultural University，2006.(in Chinese)
［19］刘晓强.宠物源大肠杆菌对氟喹诺酮类药物的多药耐药机制研究［D］.杨凌：西北农林科技大学，2012.
LIU X Q.Mechanism accounting for fluoroquinolone multidrug resistance in Escherichia coli isolated from companion animals［D］.Yangling：Northwest A&F University，2012.(in Chinese)

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Detection of Virulence Genes and Antimicrobial Resistance Analysis of Escherichia coli Isolated from Dairy Cow Mastitis in Ningxia

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